Overview

Polymerase Chain Reaction (PCR) is a widely used molecular biology technique that allows for the amplification of specific DNA sequences. This powerful method enables researchers to create millions of copies of a particular DNA segment, making it easier to study and analyze genetic material. PCR has revolutionized molecular biology and has numerous applications in research, clinical diagnostics, forensics, and more. Conventional PCR, also known as end-point PCR, involves the amplification of DNA and the subsequent detection of PCR products at the end of the reaction using methods such as gel electrophoresis. This traditional approach is widely used for various applications, including cloning, sequencing, and analysis of gene expression.

Features

Our conventional PCR master mix is meticulously formulated to deliver exceptional performance and reliability in a wide range of molecular biology applications.

· High Sensitivity  :  Engineered to detect even low-abundance DNA targets with exceptional sensitivity, ensuring reliable amplification and detection.
· Superior Specificity  :  Minimizes non-specific amplification and primer-dimer formation, resulting in clean, accurate results without background noise.
· Robust Performance  :  Provides consistent and reproducible results across a broad range of templates and conditions, including challenging samples and complex genomic DNA.
· Thermostable DNA Polymerase  :  Utilizes a high-fidelity, thermostable DNA polymerase that maintains activity at elevated temperatures, ensuring efficient and reliable amplification.
· Optimized Buffer System  :  Contains a proprietary buffer formulation that enhances enzyme activity and stability, providing optimal conditions for PCR amplification.
· Fast Cycling Capability  :  Optimized for rapid cycling protocols, reducing overall reaction time and increasing throughput without compromising performance.
· Versatility  :  Suitable for various PCR applications, including routine PCR, genotyping, cloning, and screening, offering flexibility for diverse research needs.
· Ease of Use  :  Pre-mixed and ready-to-use formulation simplifies reaction setup, reducing hands-on time and minimizing the risk of pipetting errors.
· Consistency  :  Formulated to deliver consistent results from batch to batch, ensuring reliable performance and reproducibility across experiments.
· Compatibility  :  Compatible with a wide range of thermal cyclers and PCR instruments, allowing for seamless integration into existing laboratory workflows.

With these advanced features, our conventional PCR master mix provides researchers with a powerful tool for precise and reliable DNA amplification in molecular
biology research and diagnostic applications.

Product Selection

· Standard Taq DNA Polymerase Master Mix  :  Ideal for routine PCR applications, offering high efficiency and reliability.
· Hot-Start Taq DNA Polymerase Master Mix  :  Prevents non-specific amplification and primer-dimer formation, enhancing the specificity of PCR reactions.
· High-Fidelity Polymerases Master Mix  :  For cloning applications requiring precise and accurate DNA replication.

Applications

Our conventional PCR master mix is a versatile tool designed to facilitate a wide range of molecular biology applications. Key applications

· Gene Amplification  :  Amplify specific DNA sequences for cloning, sequencing, and functional analysis.
· Genotyping  :  Identify genetic variations such as single nucleotide polymorphisms (SNPs) and insertions/deletions (indels).
· DNA Sequencing  :  Prepare templates for Sanger sequencing and other sequencing methodologies.
· Gene Expression Analysis  :  Quantify gene expression levels using relative or absolute quantification methods.
· Molecular Diagnostics  :  Detect pathogens, mutations, or genetic markers associated with diseases.
· Forensic Analysis  :  Analyze DNA samples for forensic identification and criminal investigations.
· Environmental Monitoring  :  Detect and quantify microbial populations in environmental samples.
· Food Safety Testing  :  Screen food products for the presence of pathogens or genetically modified organisms (GMOs).
· Quality Control  :  Verify the integrity and purity of DNA samples in laboratory workflows.
· Microbial Detection  :  Identify and quantify microbial contaminants in pharmaceutical and biotechnological products.
· Species Identification  :  Discriminate between closely related species using species-specific markers.
· Viral Load Quantification  :  Measure the concentration of viral nucleic acids in clinical samples for viral load monitoring.
· Mutation Screening  :  Screen samples for specific mutations associated with inherited diseases or cancer.

Our conventional PCR master mix provides researchers with a reliable and efficient tool for a wide range of molecular biology applications, enabling discoveries in research, diagnostics, and beyond.

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